Parahydrogen Induced Polarization of 1-13C-Phospholactate-d2 for Biomedical Imaging with >30,000,000-fold NMR Signal Enhancement in Water
نویسندگان
چکیده
The synthetic protocol for preparation of 1-(13)C-phosphoenolpyruvate-d2, precursor for parahydrogen-induced polarization (PHIP) of 1-(13)C-phospholactate-d2, is reported. (13)C nuclear spin polarization of 1-(13)C-phospholactate-d2 was increased by >30,000,000-fold (5.75 mT) in water. The reported (13)C polarization level approaching unity (>15.6%), long lifetime of (13)C hyperpolarized 1-(13)C-phospholactate-d2 (58 ± 4 s versus 36 ± 2 s for nondeuterated form at 47.5 mT), and large production quantities (52 μmoles in 3 mL) in aqueous medium make this compound useful as a potential contrast agent for the molecular imaging of metabolism and other applications.
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